Helicobacter cyclurae sp. Nov., Isolated From Endangered Blue Iguanas (Cyclura lewisi)

Blue iguanas ( Cyclura lewisi ) are endangered reptiles found only on Grand Cayman. Previously, DNA for a novel Helicobacter species GCBI1 was detected in sick and dead iguanas. In the current study, fecal cloacal swab samples were obtained from 25 Through molecular microbiological techniques, cultured feces characterized, whom we propose name cyclurae . This helicobacter had prevalence of 56% by PCR 20% culture analyzed. The type strain MIT 16-1353 catalase, oxidase, gamma-glutamyl transpeptidase positive. By electron microscopy, H. has curved rod morphology single sheathed polar flagellum. Phylogenetic analysis using 16S rRNA, gyr B, hsp 60 indicated that these strains most closely related to sp. 12502256-12 previously isolated lizards. 1.91-Mb genome with GC content 33.37%. There 1,969 genes four notable virulence genes: high temperature requirement-A protein-secreted serine protease, transpeptidase, fibronectin/fibrinogen binding protein, neutrophil-activating protein. Whole-genome phylogeny, average nucleotide identity, digital DNA–DNA hybridization confirmed is species, first characterized blue